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National Repository of Grey Literature 5 records found  Search took 0.01 seconds. 
Ordered and disordered arrays of colloidal nanoparticles for biomolecule detection
 Ligmajer, Filip ; Rezek, Bohuslav (referee) ; Kolíbal, Miroslav (advisor)
This thesis deals with guided self-assembly of gold nanoparticles from their colloidal solutions onto silicon substrates and possible employment of nanoparticles for detection of biomolecules. It was found that by adjustment of solution pH and surface chemistry modification by means of electron beam irradiation it is possible to facilitate nanoparticle deposition to patterns with almost single particle precision. Spectroscopic ellipsometry was then employed in analysis of self-assembled layers of nanoparticles and its combination with a theory of effective medium approximation has proven the ability to assess nanoparticle dimensions and volume fractions. By experiments with thiolated oligonucleotides it has been shown that using ellipsometry one can detect even with very subtle changes in nanoparticle environment caused by biomolecules, thus promising its possible use in the field of biodetection.
Detailed record
Deposition of Metal Nanoparticles from Solution and Study of Their Optical Properties
 Ligmajer, Filip ; Urbánek, Michal (referee) ; Kolíbal, Miroslav (advisor)
This bachelor's thesis deals with metal nanoparticles, their deposition from colloidal solutions to a silicon surface and investigation of their optical properties. It is demonstrated that due to the modification of these solutions by hydrofluoric or hydrochloric acid there is a distinct increase in nanoparticle concen\-tration on the surface. The optical properties of prepared samples were measured by spectroscopic ellipsometry and reflectometry.
Detailed record
Ordered and disordered arrays of colloidal nanoparticles for biomolecule detection
 Ligmajer, Filip ; Rezek, Bohuslav (referee) ; Kolíbal, Miroslav (advisor)
This thesis deals with guided self-assembly of gold nanoparticles from their colloidal solutions onto silicon substrates and possible employment of nanoparticles for detection of biomolecules. It was found that by adjustment of solution pH and surface chemistry modification by means of electron beam irradiation it is possible to facilitate nanoparticle deposition to patterns with almost single particle precision. Spectroscopic ellipsometry was then employed in analysis of self-assembled layers of nanoparticles and its combination with a theory of effective medium approximation has proven the ability to assess nanoparticle dimensions and volume fractions. By experiments with thiolated oligonucleotides it has been shown that using ellipsometry one can detect even with very subtle changes in nanoparticle environment caused by biomolecules, thus promising its possible use in the field of biodetection.
Detailed record
Deposition of Metal Nanoparticles from Solution and Study of Their Optical Properties
 Ligmajer, Filip ; Urbánek, Michal (referee) ; Kolíbal, Miroslav (advisor)
This bachelor's thesis deals with metal nanoparticles, their deposition from colloidal solutions to a silicon surface and investigation of their optical properties. It is demonstrated that due to the modification of these solutions by hydrofluoric or hydrochloric acid there is a distinct increase in nanoparticle concen\-tration on the surface. The optical properties of prepared samples were measured by spectroscopic ellipsometry and reflectometry.
Detailed record
The possibilities of multiple immunolabelling of biological samples in a scanning electron microscope
 MARTYKÁNOVÁ, Denisa
In this thesis I evaluate the Field Emission Scanning Electron Microscope (FESEM) as a suitable instrument for immunolocalization of cell components by specific binding of the antigen - antibody labelled with colloidal gold. The aim of this thesis is to find in the literature the techniques of the specimen?s preparation for immunolabelling, properties of the markers and the possibilities of their use for multiple immunolabeling of biological samples.
Detailed record

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